Developing a robust quantitation assay for monoclonal. Fortebio acquired by sartorius ag apr 30, 2020 on april 30, 2020, sartorius ag acquired life science company fortebio, the former biologics business unit of molecular devices. Controlled access with multiple user privileges adminis trator, developer. The instrument should be on for at least 1 hour prior to running an experiment. Fortebio octet qk labelfree analyzer with computer and. Using the more complex models available in the octet analysis software can help troubleshoot more complex curves and optimize the design of kinetics experiments fortebio offers different chemistry biosensors for off theshelf use octet can use up to 16 biosensors for scouting experiments, and each sensor is independent. If you do not follow these instructions carefully, you may get the wrong forteo dose.
It also provides documented verification that the octet instrument operates as intended at the user site by inspecting the critical components. Octet is designed to accelerate antibody development by generating realtime quantitative and kinetics results quickly and easily. Significantly easier, faster and better characterization of drug candidates is possible, providing greater value in drug development applications where existing methods have limitations in throughput, performance and cost. Utilizes biolayer interferometry bli to enable label free, realtime analysis of protein. Octet red384 getting started introduction the octet red384 is an instrument for biolayer interferometry bli, an optical technique for measuring macromolecular interactions by analyzing interference patterns of white light reflected from the surface of a biosensor tip. For each experiment, youll need to bring fortebio biosensors, at least 2 black. Additionally, we determined the affinity of 2h2 binding to med25 using the fortebio octet system, which applies the optical interference theory and produces highly accurate and reproducible data. Octet htx system for high throughput detection and fine resolution analysis for quantitation and kinetic characterization, using labelfree, realtime analysis. The octet htx and octet red384 utilize fortebios dip and read technology with the read head dipping into samples in a plate format. Instrument qualification the iqoq validation manual and testing kit provides a stepbystep procedure for the installation qualification iq and operation qualification oq of the blitz system. The latest release, version 11, is available for use on all octet systems. General requirement seeking contractor to provide all labor, tools, material, parts, equipment, supervision, software, license, certification, transportation, management, and personnel required for a preventive maintenance on government equipment octet red384 system, located in bldg.
The system has been developed to operate reliably in a regulated environment. The analysis software needs baseline, association and dissociation steps consecutively to work. This spr analyzer has capabilities ranging from proteinprotein down to proteinsmall molecule and has a rapid sampling rate that enables fast and slow kinetics with low noise. Octet qke system pall fortebio 47661 fremont boulevard fremont, ca 94538 t 888. High throughput octet htx and octet red384 systems fortebio. The octet software kinetics analysis and reporting user software enabling graph exporting in a number of different formats streamlined data analysis and model fitting within origin 7. The octet htx and octet red384 utilize fortebio s dip and read technology with the read head dipping into samples in a plate format. First read the medication guide that comes inside your forteo carton. In addition, installation and qualification iqoq and performance qualification pq kits are available for gxp users.
Fortebio octet data acquisition and data analysis software is rapidly evolving to better address the growing applications and needs of our customers. From kinetics and quantitation analysis of antibodies, proteins, protein residual contaminants and other biological molecules, octet software is intuitive and easy to use. The octet red96e system is widely used in analytical core labs for protein structurefunction studies and small molecule screening campaigns. Forteo fortayo teriparatide injection user manual important. Contact information the fortebio octet red384 system is located in 3006 veterinary medicine research facility 3b vm3b. Fortebio octet qk labelfree analyzer with computer and octet software lot 400. Serial fivefold concentrations of med25 were analyzed, and each of the concentrations yielded the same results kd 8. Proteon xpr36 equipped with glc sensor chips and coupling reagents 10 mm sodium acetate ph 4. With no fluidics involved, octet systems are highly robust. Do not store your forteo pen with the needle attached. This spr analyzer has capabilities ranging from proteinprotein down to proteinsmall molecule and has a rapid sampling rate.
Exploring blocking assays using octet, proteon, and. Servswitch octet kv1701, servswitch octet kv1702, servswitch octet kv1703, servswitch octet. Analysis can be done using a single channel or up to eight channels, enabling flexibility in sample throughput based on need. The versatile 8channel octet systems provide flexibility for rapid assay development. The blitz system and blitz pro software user guide contains information on materials. The molecular devices online store is currently available to shipping addresses within the united states only. Identification of a common epitope between enterovirus 71 and. Identification of a common epitope between enterovirus 71. Keeping the needle on may let forteo leak from the pen, and air bubbles can form in the cartridge. Octet realtime drug and protein binding kinetics unit. Configure, install, and operate your blitz system and blitz pro software. User friendly software for data acquisition and analysis. On april 30, 2020, sartorius ag acquired life science company fortebio, the former biologics business unit of molecular devices.
Set up and run kinetics experiments on the blitz instrument. The octet qke has provided us with a robust and versatile platform for the measurement of antibody antigen interactions. In addition, the sample material remains available after analysis for further investigation. Lab analyzers can be used to analyze samples in a variety of settings. Accordingly, downstream processes require detailed characterization of specificity, selectivity, stability and binding rate analyses under various growth or buffer conditions to facilitate informed process decisions. Operation of fortebio molecular interactions instrument. Biomolecular binding kinetics assays on the octet platform 14 introduction direct measurement of biomolecular interactions plays an important role in biotherapeutic drug discovery and development. Biacore t200 supports the use of 96 and 384well microplates and vials.
Servswitch octet kv1701, servswitch octet kv1702, servswitch octet kv1703, servswitch octet kv1712, servswitch octet kv17, servswitch octet kv1711. Follow the directions carefully when using the forteo delivery device. View and download black box servswitch octet kv1700 user manual online. For questions or to get started, please contact unit director alexey tomilov, phd, at. The octet red96e system is designed to work reliably in regulated environments. Octet red384 getting started introduction the octet red384 is an instrument for biolayer interferometry bli, an optical technique for. Fortebio introduces the octet system technology networks. As an increasing number of research methods currently require a polymerase chain reaction pcr step, the thermocycler has become an essential tool in the modern life science laboratory. Epitope binning by fortebio octet platform when multiple antibodies have similar affinities for a target, determining whether they bind to the same or different epitopes on the target molecule becomes an inevitable step towards evaluating their individual functional potential.
Table of recommened sensor offsets by well volume from octet user manual. Both automated and manual ingel digest protocols need approximately one hour of preparation green bar, but automation frees the user from additonal handson time red bars over a seven hour period. The sample compartment of biacore t200 can be cooled down to 4c, enabling the analysis of sensitive samples in unattended runs of up to 48 h. The use of all four flow cells allows four interactions to be simultaneously monitored. Converting an elisa assay into an octet quantitation assay. These comparison tables provide a general guide to the features included in various versions of the software. From chemistry analyzers that analyze urine, blood, and plasma, to bioanalyzers that use microfluidic technology. Remember to put into the concentration of mab and antigen. Includes computer equipped with octet user software 4. Uom description 305048 system system includes octet red96 instrument, octet software v6. The htx read head provides design flexibility that allows users to analyze, 8, 16, 32, 48, or 96. Data kindly provided by maxplanckinstitute of experimental medicine in gottingen, germany see jahn et al.
Octet red96egxp system included octet red96e instrument, octet cfr software, desktop computer, lc monitor, accessory kit, iqoq kit, pq kits and oneyear warranty 1852 pack singleuse evaporation covers to extend the experiment up to 12 hours. Although the measuring the amount of bound material is done in a different way from surface plasmon resonance spr in the well known biacore instruments. Black box servswitch octet kv1700 user manual pdf download. The octet red96e system is microfluidicsfree, thereby reducing assay complexity, eliminating timeconsuming cleaning steps and cutting operating costs. Pall fortebio launches the octet k2 system 7aug2015. Converting an elisa assay into an octet quantitation assay introduction quantitation assays on the octet platform have many similarities to enzymelinked immunosorbent assays elisa. The iqoq kit provides comprehensive documentation to verify the octet instrument, accessories and computer system are supplied and installed per fortebios guidelines by certified personnel. For information on preparing samples for quantitation or kinetics experiments, please see the appropriate fortebio octet biosensor product instructions. Welcome to the fortebio octet system data acquisition user guide.
The octet red96e system detects a diverse range of bio molecules from small. Increasing access to sensitive binding interactions analysis. A food safety tester or pathogen analyzer or clinical analyzer. Octet systems enable realtime, labelfree analysis for determination of affinity, kinetics and concentration. Epitope binning by fortebio octet platform creative biolabs. Responsible for designing, developing and executing the test specification and test plans for octet pro 5. Fortebio acquisition by sartorius molecular devices. Providing technologies to accelerate therapeutics development. Pall fortebio, a leading supplier of instrumentation for accelerated drug discovery and development, has announced the launch of the octet k2 system, a new entry point into the octet line of labelfree interaction analysis. The 8channel octet systems perform quantitation of 96 samples in 32 minutes and kinetic screening of 64 samples in 1. Octet red384 getting started introduction load analyte sample. Pall fortebio octet systems european biotechnology. Summar y octet systems provide an automated solution for maximizing the efficiency of antibody detection and quantitation in biopharmaceutical development and manufacturing processes. Experience the identifiably different spectramax id3 multimode microplate reader.
Operation of fortebio molecular interactions instrument the fortebio red octet 96 instrument uses biolayer interferometry to measure binding of molecues to a protein immobilized on a probe. Fortebio offers 21 cfr part 11 software and a full line of gxp products and services as part of the octet red96e gxp package. Pall fortebio launches the octet red96e system for. The octet red384 is an instrument for biolayer interferometry bli, an optical. Fremont, ca prweb april 05, 2017 pall fortebio, the market leader in labelfree biomolecular interaction analysis across academic research, drug discovery and development, process analytics, and manufacturing quality control today announced the launch of the new octet red96e instrument. Labelfree analytical technologies such as the octet platform from pall fortebio provide a powerful means to obtain accurate information. Before you use your new forteo delivery device, please read the entire front and back of this user manual completely. Mar 03, 2015 fortebio octet qk labelfree analyzer with computer and octet software lot 400. Hudson robotics lab automation fortebio octet workcell. Servswitch octet kv1700 switch pdf manual download.
Both are performed on a solid support on which the capture molecule is immobilized and the analyte is bound from solution. For more details, please check the data analysis user guide provided by octet qk system. Biolayer interferometry bli using the octet red96e system. Fortebio octet red96 analysis of macromolecular interactions and binding kinetics data acquisition manual data analysis manual sample preparation. Protein detection and analysis tecan life sciences home. Octet qk equipped with aminereactive ar biosensor tips and coupling buffer, 100 mm 2nmorpholinoethanesulfonic acid mes, ph 5.